Human B7H1/PD-L1 ELISA Kit

Name: Human B7H1/PD-L1 ELISA Kit
Brand: ELH-B7H1
SKU: ELH-B7H1
Price: 500 USD
Availability: InStock
Rating: 93 (3 reviews)

Rating:

93 % of 100

3 Reviews

RayBio® Human B7-H1 (CD274) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Documents

As low as $500.00

Find a Distributor

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human
Accession Number	Q9NZQ7
Gene Id	29126
Gene Symbols	CD274\|B7H1\|PDCD1L1\|PDCD1LG1\|PDL1
Protein Name / Synonyms	Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274)
Quantitative/Semi-Quantitative	Quantitative
Specificity	The antibody pair provided in this kit recognizes Human B7-H1 (CD274)
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Research Area	Antibody Drug Development, Protein Therapeutics, Biomarker Detection
Sensitivity	46 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	46 pg/ml - 3000 pg/ml
Recommended Dilution (Serum/Plasma)	2 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

Risk-Free Guarantee

We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

Learn More

Amazon Gift Cards!

$5 Amazon gift card in every kit box purchased.

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELH-B7H1.pdf)

SDS (ELH-B7H1.pdf)

Excel (sandwich-elisa.xlsm)

Reviews

Write a customer review

Great product

Rating
100%

I am using the kits from RayBiotech and it easy to follow with the cell culture supernatant. You might need to try different fold of dilutions.

Review by Samia,

from Florida A&M University,

on Dec 17, 2020
Great product

Rating
100%

Great kit. Protocol easy to follow, as all other ELISA protocols from RayBiotech. Great and reproducible results.

Review by Patricia,

from Florida A&M University,

on Mar 15, 2020
Great product

Rating
80%

Kit works very well. Nice, reproducible results. The instructions can be a little confusing (example "Assay Diluent B" is labeled "Item E" and "Diluent D" is "Item K"), but as long as you read carefully, shouldn't be a problem.

Review by Susan Stephenson,

from Emory University - SOM: PEDS,

on Feb 5, 2019

Write Your Own Review

Your Rating

1 star 2 stars 3 stars 4 stars 5 stars

Display Name

Your email address is required to receive a $10 Amazon Gift Card. It will not be posted with the review. Gift cards are emailed out every Friday.

Review Title / Summary

Institution

Review (include details such as sample type used, experimental conditions, etc.)

Submit a review for this product on Biocompare.com to earn an entry into a monthly raffle for one of five $100 Amazon gift cards!

Ask a Question

Display name:

Your e-mail:*

Your message (include experimental conditions or sample type used):*

Your location:*

U.S.

Non-U.S.

Name

This field is for validation purposes and should be left unchanged.

Featured products

Human B7H1/PD-L1 ELISA Kit