RayBio® Human AXL ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

As low as $420.00
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Catalog #:
ELH-AXL
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Human
Accession Number P30530
Gene Id 558
Gene Symbols AXL|UFO
Protein Name / Synonyms Tyrosine-protein kinase receptor UFO (EC 2.7.10.1) (AXL oncogene)
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Sensitivity 2 pg/ml

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Detection Range 2 pg/ml - 2000 pg/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Hsiao F., Lin Y., Hsieh P., Chu N., Shieh Y., et al. Circulating Growth Arrest-Specific 6 Protein Is Associated With Adiposity, Systemic Inflammation, and Insulin Resistance Among Overweight and Obese Adolescents. JCEM Vol 98 Issue 2 http://dx.doi.org/10.1210/jc.2012-3179
Species:
Human
Sample Type:
Plasma
Hsiao F-C, Lin Y-F, Hsieh P-S, et al. Circulating growth arrest-specific 6 protein is associated with adiposity, systemic inflammation, and insulin resistance among overweight and obese adolescents. J Clin Endocrinol Metab. 2013;98(2):E267-E274.
Species:
Human
Sample Type:
Plasma (intra-assay and interassay coefficients of variation were <10% and <12%)
Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
Species:
Human
Sample Type:
Serum (Lupus Nephritis patients)
Farschtschi, S., Kluwe, L., Park, SJ. et al. Upregulated immuno-modulator PD-L1 in malignant peripheral nerve sheath tumors provides a potential biomarker and a therapeutic target. Cancer Immunol Immunother 69, 1307-1313 (2020). https://doi.org/10.1007/s00262-020-02548-1
Species:
Human
Sample Type:
Serum
Lee, Chien-Hsing, et al. "Plasma concentrations predict aortic expression of growth-arrest-specific protein 6 in patients undergoing coronary artery bypass grafting." PloS one 8.11 (2013): e79452.
Species:
Human
Sample Type:
Plasma
Liu YW., Yang QF., Zuo PY, et al. Elevated Serum Levels of Soluble Axl in Acute Coronary Syndrome. Am J Med Sci. 2015 Feb;349(2):124-9. doi: 10.1097/MAJ.0000000000000362
Species:
Human
Sample Type:
Serum (Acute Coronary Syndrome patients)
Bakr S., et al. Serum growth arrest specific protein 6 and its receptor (Axl) in SLE patients. The Egyptian Rheumatologist, Sept 2014.DOI:10.1016/j.ejr.2014.07.003
Species:
Human
Sample Type:
Serum
  1. Great product
    Rating
    100%
    The AXL ELISA kit by RayBiotech is a reliable and easy-to-use kit for detecting AXL protein levels with excellent sensitivity and specificity. The kit provides a broad dynamic range, making it suitable for a wide range of applications in basic research and clinical diagnostics. Overall, I highly recommend this kit for researchers looking to study the AXL signaling pathway.

    Review by

    from Brigham Young University,

    on

  2. Great product
    Rating
    100%
    The procedure was very clear and organized. They even included figures to help us understand what we needed to do. Everything was neatly packed and organized.

    Review by

    from Brigham Young University,

    on

  3. Great product
    Rating
    80%
    This is a great kit. Was able to generate a very good standard curve, and the experiment itself worked

    Review by

    from Plymouth University,

    on

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