PhosphoFlow Services
RayBiotech offers a suite of customized flow cytometry services, including phosphorylation flow cytometry analysis on your population of cells. This is a unique flow technique that allows for the analysis and quantification of the phosphorylation status of specific proteins within individual and specific cell populations heterogeneous sample. Phosphorylation is a crucial post-translational modification of proteins that plays a central role in cell signaling, regulating various cellular processes such as cell growth, differentiation, and response to external stimuli. The data generated by the phosphoflow can be used to quantify the lack or extent of phosphorylation, identify cell populations with specific phosphorylation patterns, and gain insights into cellular signaling events.
Phosphoflow is a valuable tool in cell biology and immunology research because it allows scientists to study cellular responses to various stimuli at a single-cell level. Such analysis in conjunction with other flow or related data can help to identify intricate signaling pathways and regulatory mechanisms within cells. Whether you are interested in immune responses profiles, cancer cell signaling, or other biological processes where protein phosphorylation is critical, RayBiotech is here to help.
Advantages of Phosphoflow cytometry over other assays:
- Single Cell Resolution: Since the analysis on the cytometer is individual cells, one at a time, you get single cell data on every cell that passes through. This provides insights into the heterogeneity of cell populations, variation in signaling within the group, as well as at the individual cell level.
- Multiplexing: Since flow cytometers can measure anywhere from 1 to 20+ fluorochromes at a time, multiple antibodies with different fluorophors can be used simultaneously. This enables the gating of multiple cell surface markers to delineate specific cell populations as well as multiple phosphorylation events in the same cell, providing a more comprehensive view of cellular signaling pathways.
- High-Throughput: Flow cytometers can analyze thousands of individual cells per second, making the technique one of the fastest single cell tools available. This allows researchers to quickly examine large cell populations and obtain statistically robust data.
Discuss your project requirements
A multicolor flow panel looking at the phosphorylation levels of ERK1/2, MAPK, STAT3, and STAT6 in T cells, B cells, and Monocytes after specific stimuli. Cells were from red blood cell removed Human PBMCS
