RayBio® Mouse Insulin ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended as an anticoagulant for use in this assay.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Catalog #:
ELM-Insulin
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit, 10 Plate Kit
Species Mouse
Accession Number P01325
Gene Id 16333
Gene Symbols INS1|INS-1
Protein Name / Synonyms Insulin-1 [Cleaved into: Insulin-1 B chain Insulin-1 A chain]
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA antibody pair detects mouse, human, rat, porcine and bovine Insulin.
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Sensitivity 5 µIU/ml

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Detection Range 5 µIU/ml - 400 µIU/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Target Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Uday Chandrika, K., Tripathi, R., Kameshwari, Y. et al. Refunctionalization of Decellularized Organ Scaffold of Pancreas by Recellularization: Whole Organ Regeneration into Functional Pancreas. Tissue Eng Regen Med 18, 99-112 (2021). https://doi.org/10.1007/s13770-020-00296-y
Species:
Mouse
Sample Type:
Serum
Liu Z, Ding Z, Lynch EC, McCauley N, Zhou Y, Zhang KK, Xie L. Pregestational diet transition to normal-fat diet avoids the deterioration of pancreatic ?-cell function in male offspring induced by maternal high-fat diet. J Nutr Biochem. 2020 Dec;86:108495. doi: 10.1016/j.jnutbio.2020.108495. Epub 2020 Sep 16. PMID: 32949717; PMCID: PMC7643480.
Species:
Mouse
Sample Type:
Plasma
Arha, Deepti, et al. "Isoalantolactone derivative promotes glucose utilization in skeletal muscle cells and increases energy expenditure in db/db mice via activating AMPK-dependent signaling." Molecular and cellular endocrinology 460 (2018): 134-151.
Species:
Mouse
Sample Type:
Cell Lysate (rat skeletal muscle cells)
Zhou Y, Liu Z, Lynch EC, He L, Cheng H, Liu L, Li Z, Li J, Lawless L, Zhang KK, Xie L. Osr1 regulates hepatic inflammation and cell survival in the progression of non-alcoholic fatty liver disease. Lab Invest. 2020 Oct 1. doi: 10.1038/s41374-020-00493-2. Epub ahead of print. PMID: 33005011.
Species:
Mouse
Sample Type:
Serum
Abdel-Latif M. Diethylcarbamazine citrate ameliorates insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation. Int Immunopharmacol. 2015 Dec;29(2):607-12. doi: 10.1016/j.intimp.2015.09.021.
Species:
Mouse
Sample Type:
Serum (outbred swiss albino mice treated with Diethylcarbamazine citrate for insulin resistence)
Mandaliya DK, Patel S, Seshadri S. The Combinatorial Effect of Acetate and Propionate on High-Fat Diet Induced Diabetic Inflammation or Metaflammation and T Cell Polarization. Inflammation. 2021 Feb;44(1):68-79. doi: 10.1007/s10753-020-01309-7. PMID: 32978698.
Species:
Mouse
Sample Type:
Serum
Eo H., Jeon Yj., Lee M., et al. Brown Alga Ecklonia cava Polyphenol Extract Ameliorates Hepatic Lipogenesis, Oxidative Stress, and Inflammation by Activation of AMPK and SIRT1 in High-Fat Diet-Induced Obese Mice. J Agric Food Chem. 2014 Dec 22.
Species:
Mouse
Sample Type:
Plasma (High-fat diet mice)
Species:
Mouse
Sample Type:
Plasma
Othman, Azza I., et al. "Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats." Biomedicine & Pharmacotherapy 94 (2017): 362-373.
Species:
Rat
Sample Type:
Serum (diabetes)
Majeed C., Al-Shammari A., Yausif E., et al. BM-MSCs Differentiated Insulin-Producing Cells Produce More Insulin in Presence of EGF than of FGF. Stem Cell Discovery 2015, 5, 33-39. http://dx.doi.org/10.4236/scd.2015.54004
Species:
Mouse
Sample Type:
Conditioned Media (BM-MSC cells)
  1. Exemplary
    Rating
    100%
    An incredible kit that worked well with our aged mice comparisons.

    Review by

    from Mayo Clinic,

    on

  2. Insulin secretion
    Rating
    100%
    Insulin secretion from MIN 6 cells was evaluated and the standards gave an excellent reading.

    Review by

    from Sri Anjaneya Institute ,

    on

  3. Excellent Assay
    Rating
    100%
    The assay was easy to understand and the values were comparable to another kit we ran. I have no complaints.

    Review by

    from Pennsylvania State University,

    on

  4. Great product
    Rating
    100%
    The kit worked perfectly well for all samples. Plasma samples from obese mice were above detection limit in first trial. Diluting them further worked well the next time.

    Review by

    from Case Western Reserve University,

    on

  5. Great product
    Rating
    100%
    Excellent

    Review by

    on

  6. Great product
    Rating
    80%
    Purchased the mouse insulin ELISA kit recently and it detected all plasma samples except few samples from obese mice. They are over the high detection range. Works great and will re-purchase in the future.

    Review by

    from University of Iowa,

    on

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