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    Mouse IgE ELISA Kit

    Rating:
    100 % of 100
    4  Reviews
    1 Citations

    RayBio® Mouse IgE ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

    Documents
    As low as $420.00
    In stock
    Only %1 left
    Catalog #:
    ELM-IgE
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Mouse
    Quantitative/Semi-Quantitative Quantitative
    Specificity This ELISA antibody pair detects mouse IgE. Other species not determined.
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 0.14 ng/mL

    Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.
    Detection Range 0.14 ng/mL - 100 ng/mL
    Recommended Dilution (Serum/Plasma) 10,000 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage ≤-20°C

    Risk-Free Guarantee

    We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

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    $5 Amazon gift card in every kit box purchased.

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Citations

    Matsushita A., Seike M., Okawa H., et al. Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis. Exp Dermatol. 2012 Sep;21(9):714-5. doi: 10.1111/j.1600-0625.2012.01559.x.
    Species:
    Mouse
    Sample Type:
    Tissue Lysate (Skin lysates from trinotriochlorobenzene TNCB treatment, allergic dermatitis model)
    View Publication

    Reviews

    Write a customer review
    1. Excellent ELISA Kit That's Actually Affordable
      Rating
      100%
      RBT's Mouse IgE ELISA Kit appears to be nearly identical in it's reagents and design to another popular kit out there that sells for hundreds more. This kit has been great not only because it's more economical, but samples are just as reliably read at 450nm on our Synergy2 plate reader, CV%s are tight, and inter-plate reliability seems to be just as good (if not better) than that other more expensive kit company. This is my new go-to ELISA kit :)

      Review by

      from North Carolina State University,

      on

    2. Consistent Kit
      Rating
      100%
      Used this kit to confirm disease models with consistent success.

      Review by

      from North Carolina State University,

      on

    3. Great product
      Rating
      100%
      nice kit, helpful to my experiment.

      Review by

      from Yale University,

      on

    4. Great product
      Rating
      100%
      We used this kid in Mouse serum of animal treated with a human IgG therapeutic antibody

      Review by

      from BTSresearch,

      on

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