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    Human Osteoactivin ELISA Kit

    Rating:
    100 % of 100
    3  Reviews
    2 Citations

    RayBio® Human Osteoactivin (GPNMB) ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

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    As low as $500.00
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    Catalog #:
    ELH-Osteoactivin
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human
    Accession Number Q14956
    Gene Id 10457
    Gene Symbols GPNMB|NMB|HGFIN|UNQ1725/PRO9925
    Protein Name / Synonyms Transmembrane glycoprotein NMB (Transmembrane glycoprotein HGFIN)
    Quantitative/Semi-Quantitative Quantitative
    Specificity This antibody pair detects human Osteoactivin. It show less than 20% cross-reactivity with recombinant mouse Osteoactivin.
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 45 pg/ml

    Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.
    Detection Range 45 pg/ml - 12,000 pg/ml
    Recommended Dilution (Serum/Plasma) 10 - 110 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage ≤-20°C

    Risk-Free Guarantee

    We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

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    $5 Amazon gift card in every kit box purchased.

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Citations

    Brekk OR, Honey JR, Lee S, Hallett PJ, Isacson O. Cell type-specific lipid storage changes in Parkinson's disease patient brains are recapitulated by experimental glycolipid disturbance. Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27646-27654. doi: 10.1073/pnas.2003021117. Epub 2020 Oct 15. PMID: 33060302.
    Species:
    Human
    Sample Type:
    Tissue Lysate (Brain homogenates)
    View Publication
    Brekk OR, Honey JR, Lee S, Hallett PJ, Isacson O. Cell type-specific lipid storage changes in Parkinson's disease patient brains are recapitulated by experimental glycolipid disturbance. Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27646-27654. doi: 10.1073/pnas.2003021117. Epub 2020 Oct 15. PMID: 33060302.
    Species:
    Human
    Sample Type:
    Tissue Lysate (human Substantia Nigra homogenates)
    View Publication

    Reviews

    Write a customer review
    1. Great ELISA Kit for GPNMB
      Rating
      100%
      Worked well with CSF samples. Got the expected results.

      Review by

      from Nationwide Children's Hospital,

      on

    2. Great product
      Rating
      100%
      Used this kit successfully with several immortalized cell lines, iPSC-derived cells, and mouse brain lysates so far with very good results that correlated with GPNMB RNA data. Limit of detection has consistently been around 35pg/mL.

      Review by

      from Biogen,

      on

    3. Great product
      Rating
      100%
      We especially appreciated how all the reagents were clearly labeled "A, B, C" etc. to minimize confusion. Worth it!

      Review by

      from McLean Hospital,

      on

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