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Custom Human Phospho-ATF2 (Thr69/71) and Total ATF2 Cell-Based ELISA Kit
RayBio® Human Phospho-ATF2 (Thr69/71) and Total ATF2 Cell-Based ELISA Kit. This assay semi-quantitatively measures ATF2 phosphorylated at Threonine-69/71 as well as total ATF2 in adherent cell lines.
Product Description
Specifications
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species
Human
Accession Number
P15336|P16951
Gene Id
1386
Gene Symbols
ATF2|CREB2|CREBP1
Protein Name / Synonyms
Cyclic AMP-dependent transcription factor ATF-2 (cAMP-dependent transcription factor ATF-2) (EC 2.3.1.48) (Activating transcription factor 2) (Cyclic AMP-responsive element-binding protein 2) (CREB-2) (cAMP-responsive element-binding protein 2) (HB16) (Histone acetyltransferase ATF2) (cAMP response element-binding protein CRE-BP1)
Quantitative/Semi-Quantitative
Semi-Quantitative
Specificity
This ELISA kit recognizes Human ATF2 phosphorylated at site Threonine-69/71 as well as total ATF2.
Compatible Sample Types
Adherent Cell Culture
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Cell-based
Research Area
Post-Translational Modifications, Phosphorylation, MAPK Signaling, PI3K-AKT Signaling, TGF-beta Signaling
Estimated Lead Time
4-6 weeks
Shipping Type
Blue ice
Storage
-20°C
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
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$5 Amazon gift card in every kit box purchased.
Product Features Rapidly measure phosphorylated protein in adherent cell lines Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose) No sample lysis is needed Compatible with a standard ELISA plate reader Application Notes Kit Components
Uncoated 96-well Strip Microplate Wash Buffers Fixing Solution Quenching Buffer Blocking Buffer Anti-phospho antibody Anti-pan antibody HRP-Conjugated Secondary Antibody TMB One-Step Substrate Stop Solution Other Materials Required
Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm Protocol Outline
Seed 10,000-30,000 cells into each well and incubate overnight. Apply various treatment, inhibitors or activators according to manufacturer's instructions. Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking. Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT. Add 200 µl of Blocking Solution and incubate for 1 h at 37°C. Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT. Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Storage/Stability Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment.
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