The store will not work correctly in the case when cookies are disabled.
JavaScript seems to be disabled in your browser.
For the best experience on our site, be sure to turn on Javascript in your browser.
RayBio® Human SOD1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species
Human
Accession Number
P00441
Gene Id
6647
Gene Symbols
SOD1
Protein Name / Synonyms
Superoxide dismutase [Cu-Zn] (EC 1.15.1.1) (Superoxide dismutase 1) (hSod1)
Quantitative/Semi-Quantitative
Quantitative
Specificity
This ELISA antibody pair recognizes Human SOD1.
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Sandwich-based
Sensitivity
49 pg/mlNeed more sensitivity? Check out the new BIQ-ELISA™ kit for this target . Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.
Detection Range
49 pg/ml - 12000 pg/ml
Recommended Dilution (Serum/Plasma)
150 fold
Estimated Lead Time
1-2 business days
Shipping Type
Blue ice
Storage
-20°C
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
Learn More
Amazon Gift Cards!
$5 Amazon gift card in every kit box purchased.
Product Features Additional Notes
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes Kit Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate Other Materials Required
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
Storage/Stability The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Great product
Simple and straightforward kit. I used this for urine samples in a 1:5 dilution.
Review by
Ryan,
from
University of Houston,
on
Jun 16, 2022
Great product
We use for cervical cancer ELISA. Protocol is super easy and student friendly. It gives amazing result without any background. Get results in 1:100 dilution.
Review by
Aalekhya Sharma Biswas,
from
University of houston,
on
Jun 7, 2022
Great product
The ELISA kit worked well. The instructions were clear and easy to follow. Would recommend this kit to other researchers.
Review by
Jennifer Taggart,
from
Athersys, Inc,
on
May 24, 2018
Sign up to get promotions on your favorite research tools and research updates.
