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    Human Gas 6 ELISA Kit

    Rating:
    100 % of 100
    3  Reviews

    RayBio® Human Gas 6 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

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    As low as $440.00
    In stock
    Only %1 left
    Catalog #:
    ELH-GAS6
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human
    Accession Number Q14393
    Gene Id 2621
    Gene Symbols GAS6|AXLLG
    Protein Name / Synonyms Growth arrest-specific protein 6 (GAS-6) (AXL receptor tyrosine kinase ligand)
    Quantitative/Semi-Quantitative Quantitative
    Specificity This ELISA antibody pair recognizes Human Gas 6.
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 0.04 ng/ml

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    Detection Range 0.041 ng/ml - 30 ng/ml
    Recommended Dilution (Serum/Plasma) 2 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage ≤-20°C

    Risk-Free Guarantee

    We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

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    $5 Amazon gift card in every kit box purchased.

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Reviews

    Write a customer review
    1. Tried and True
      Rating
      100%
      This is not the first time we have ordered this product, nor will it be the last. Thank you RayBiotech for your assistance in furthering our research,

      Review by

      from University of Cincinnati,

      on

    2. Great product
      Rating
      100%
      The GAS6 ELISA kit is a high-quality kit with exceptional sensitivity and specificity for detecting GAS6 protein levels in biological samples. It is user-friendly, with a broad dynamic range suitable for a variety of applications. Overall, I highly recommend this kit for researchers studying GAS6 and its functions.

      Review by

      from Brigham Young University,

      on

    3. Great product
      Rating
      100%
      The procedure was very clear and well organized. There was no confusion whatsoever about what we needed to do. The packaging was neat, clean, and organized as well.

      Review by

      from Brigham Young University,

      on

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