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    Human C3a ELISA Kit

    Rating:
    67 % of 100
    3  Reviews
    1 Citations

    RayBio® Human C3a ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

    Documents
    As low as $420.00
    In stock
    Only %1 left
    Catalog #:
    ELH-C3a
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human
    Accession Number P01024
    Gene Id 718
    Gene Symbols C3|CPAMD1
    Protein Name / Synonyms Complement C3 (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1) [Cleaved into: Complement C3 beta chain C3-beta-c (C3bc) Complement C3 alpha chain C3a anaphylatoxin Acylation stimulating protein (ASP) (C3adesArg) Complement C3b alpha' chain Complement C3c alpha' chain fragment 1 Complement C3dg fragment Complement C3g fragment Complement C3d fragment Complement C3f fragment Complement C3c alpha' chain fragment 2]
    Quantitative/Semi-Quantitative Quantitative
    Specificity This ELISA antibody pair recognizes Human C3a.
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 63 pg/ml

    Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.
    Detection Range 63 pg/ml - 4000 pg/ml
    Recommended Dilution (Serum/Plasma) 40,000 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage -20°C

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Citations

    Hu X, Feng J, Deng S, Tang J, Liao Z, Luo L, Luo L, Meng T, Gong G, Li X. Anaphylatoxins enhance Th9 cell recruitment via the CCL20-CCR6 axis in IgA nephropathy. J Nephrol. 2020 Oct;33(5):1027-1036. doi: 10.1007/s40620-020-00708-1. Epub 2020 Feb 8. PMID: 32036611.
    Species:
    human
    Sample Type:
    Serum, Tissue Lysate
    View Publication

    Reviews

    Write a customer review
    1. Great product
      Rating
      60%
      Urine was run at 10-fold and 20-fold. Half the control samples were in range and half were BLQ at both dilutions. Most of our experimental samples (75%) were in range at 10-fold.Plasma samples were all in range when run at 1000-fold and 3000-fold.

      Review by

      from University of Michigan,

      on

    2. Great product
      Rating
      80%
      A very sensitive assay and I performed a nanoparticle-based serum analysis using this kit.

      Review by

      from Northwestern University,

      on

    3. Great product
      Rating
      60%
      Works at 1:10 dilution for human CSF

      Review by

      from University of Houston,

      on

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